Congenital heart diseases are the most common class of birth defect. However, their cause is often unknown.

Our laboratory is interested in the underlying molecular mechanisms of congenital heart disease (CHD). To achieve our goals we are using various approaches including experimental embryology, genetics, and transcriptomic experiments.

Our goals are to better understand the biological processes controlling the development of mouse and human hearts in order to identify and treat the causes of cardiac diseases. We are specifically interested in the arterial and venous poles as well as in valve development, as they are affected in the majority of CHD.

Our research focuses on the molecular programs controlling cell fate decisions between distinct embryonic cell lineages, derivatives of the neural crest and mesoderm, in the early heart. Our work over the past few years has established a novel role for a number of genes during cardiovascular development and identified several candidate genes for less-studied CHD such as bicuspid aortic valve or syndromic heart anomalies in rare malformation syndromes.



Our work over the past few years has established the role of retinoic acid in defining the boundary of the heart fields during early development. Inhibition of retinoic acid signaling leads to expansion of cardiac progenitors. We have also shown that anterior Hox genes, as downstream targets of retinoic acid, are expressed in distinct domains of the second heart field contributing to the outflow tract and atria. Using mutant mouse embryos, we have recently demonstrated that Hoxb1 regulates proliferation and differentiation of second heart field progenitors and genetically interacts with Hoxa1 during cardiac outflow tract development. The role of retinoic acid signal and Hox genes in the patterning of the second heart field is being investigated.

Finally, elucidating the mechanisms involved in the restriction of cardiac progenitors will lead to a better understanding of CHDs and will ultimately lead to the development of novel therapies aimed at healing impaired human hearts.

Anomalies of heart valves, including bicuspid aortic valve (BAV), are some of the commonest CHDs. Extracellular matrix changes occur in many valvular diseases. However, the molecular mechanisms leading to these pathologies are poorly understood. We have recently discovered the role of the zinc finger transcription factor Krox20 (Egr2) during heart valve development in mice. Loss of Krox20 function leads to defective aortic valve structure associated with aortic dysfunction. Functional promoter analysis demonstrated that Krox20 regulates the fibrilar collagens Col1a1 and Col3a1 genes during the remodeling of aortic valves. We continue our studies to uncover the contribution of different lineages in valve development and disease. We are now using state-of-the-art genetic technologies including next generation whole exome sequencing with the goal of discovering new genes in aortic valve disease such as BAV.

Inserm
AFM-Telethon
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FAVA-Multi
FFC
HuDeCa


Bernheim, S.  et al. 2023

Identification of Greb1l as a genetic determinant of crisscross heart in mice showing torsion of the heart tube by shortage of progenitor cells

Despite their burden, most congenital defects remain poorly understood, due to lack of knowledge of embryological mechanisms. Here, we identify Greb1l mutants as a mouse model of crisscross heart....
Dev Cell - issue: - volume: 23 - pages: 00493-8.

Wanert, C.  et al. 2023

Genetic profile and genotype-phenotype correlations in childhood cardiomyopathy

Background: Genetic cardiomyopathy is a rare disease in childhood. Aims: To analyse clinical and genetic aspects of a paediatric cardiomyopathy population, and to establish genotype-phenotype...
Arch Cardiovasc Dis. - issue: 6-7 - volume: 116 - pages: 309-315.

Argiro, L.  et al. 2023

Cardiopharyngeal Mesoderm specification into cardiac and skeletal muscle lineages in gastruloids

Cardiopharyngeal mesoderm contributes to the formation of the heart and head muscles. However, the mechanisms governing cardiopharyngeal mesoderm specification remain unclear. Indeed, there is a lack...
BioRxiv - issue: - volume: - pages: .

Odelin, G.  et al. 2023

Variations in the poly-histidine repeat motif of HOXA1 contribute to bicuspid aortic valve in mouse and zebrafish

Bicuspid aortic valve (BAV), the most common cardiovascular malformation occurs in 0.5-1.2% of the population. Although highly heritable, few causal mutations have been identified in BAV patients....
Nat Commun - issue: 14 - volume: - pages: 1543.

Jaouadi, H.  et al. 2023

Expanding the phenome and variome of the ROBO‑SLIT pathway in congenital heart defects: toward improving the genetic testing yield of CHD

Background Recent studies have shown the implication of the ROBO-SLIT pathway in heart development. Within this study, we aimed to further assess the implication of the ROBO and SLIT genes mainly in...
J Transl Med - issue: 21 - volume: - pages: 160.

Petolat, E.  et al. 2023

Prognostic value of forward flow indices in primary mitral regurgitation due to mitral valve prolapse

Background: Degenerative mitral regurgitation (DMR) due to mitral valve prolapse (MVP) is a common valve disease associated with significant morbidity and mortality. Timing for surgery is debated for...
Front Cardiovasc Med - issue: - volume: - pages: .

Zaffran, S.  et al. 2023

Calcium Handling in Inherited Cardiac Diseases: A Focus on Catecholaminergic Polymorphic Ventricular Tachycardia and Hypertrophic Cardiomyopathy

Calcium (Ca2+) is the major mediator of cardiac contractile function. It plays a key role in regulating excitation-contraction coupling and modulating the systolic and diastolic phases. Defective...
Int J Mol Sci - issue: 24 - volume: 4 - pages: 3365.

Lesieur, E.  et al. 2023

Prenatal screening and diagnosis of pulmonary artery anomalies: a review

Congenital pulmonary vascular anomalies are rare. Antenatal diagnosis of these vascular anomalies requires a good knowledge of fetal cardiac anatomy because clinical presentations are variable. In...
Ultrasound Obstet Gynecol - issue: - volume: 61 - pages: 445–457.

Jaouadi, H.  et al. 2022

SCN5A Variants as Genetic Arrhythmias Triggers for Familial Bileaflet Mitral Valve Prolapse

Mitral valve prolapse (MVP) is a common valvular heart defect with variable outcomes. Several studies reported MVP as an underestimated cause of life-threatening arrhythmias and sudden cardiac death...
Int J Mol Sci - issue: 23 - volume: 22 - pages: 14447.

Lin, X.  et al. 2022

Mesp1 controls the chromatin and enhancer landscapes essential for spatiotemporal patterning of early cardiovascular progenitors.

The mammalian heart arises from various populations of Mesp1-expressing cardiovascular progenitors (CPs) that are specified during the early stages of gastrulation. Mesp1 is a transcription factor...
Nat Cell Biol - issue: - volume: - pages: Epub ahead of print.

Kraoua, L.  et al. 2022

Molecular autopsy and clinical family screening in a case of sudden cardiac death reveals ACTN2 mutation related to hypertrophic/dilated cardiomyopathy and a novel LZTR1 variant associated with Noonan syndrome.

Background: Genetic cardiac diseases are the main trigger of sudden cardiac death (SCD) in young adults. Hypertrophic cardiomyopathy (HCM) is the most prevalent cardiomyopathy and accounts for 0.5 to...
Mol Genet Genomic Med - issue: 7 - volume: 10 - pages: e1954.

Jaouadi, H.  et al. 2022

Identification of non-synonymous variations in ROBO1 and GATA5 genes in a family with bicuspid aortic valve disease

Bicuspid aortic valve (BAV) is the most common congenital heart defect with a high index of heritability. Patients with BAV have different clinical courses and disease progression. Herein, we report...
J Hum Genet - issue: 67 - volume: 9 - pages: 515-518.

Jaouadi, H.  et al. 2022

Dilated-Left Ventricular Non-Compaction Cardiomyopathy in a Pediatric Case with SPEG Compound Heterozygous Variants.

Left Ventricular Non-Compaction (LVNC) is defined by the triad prominent myocardial trabecular meshwork, thin compacted layer, and deep intertrabecular recesses. LVNC associated with dilation is...
Int J Mol Sci - issue: 9 - volume: 23 - pages: 5205.

Lescroart, F.  et al. 2022

Single Cell Approaches to Understand the Earliest Steps in Heart Development

Purpose of review: Cardiac progenitors are the building blocks of the heart. Our knowledge, on how these progenitors build the heart, has considerably increased over the last two decades with the...
Curr Cardiol Rep - issue: - volume: - pages: .

Jaouadi, H.  et al. 2022

Identification of two variants in AGRN and RPL3L genes in a patient with catecholaminergic polymorphic ventricular tachycardia suggesting new candidate disease genes and digenic inheritance

Catecholaminergic polymorphic ventricular tachycardia (CPVT) is an arrhythmogenic syndrome characterized by life-threatening arrhythmias, a normal resting electrocardiogram and the absence of overt...
Clin Case Rep - issue: 10 - volume: 2 - pages: e05339.

Haniffa, M.  et al. 2021

Human Developmental Cell Atlas: milestones achieved and the roadmap ahead

The Human Developmental Cell Atlas (HDCA), as part of the Human Cell Atlas, aims to generate a comprehensive reference map of cells during development. This detailed study of development will be...
- issue: - volume: - pages: in review.

Khasawneh, R.  et al. 2021

Msx1 haploinsufficiency modifies the Pax9-deficient cardiovascular phenotype.

Background Successful embryogenesis relies on the coordinated interaction between genes and tissues. The transcription factors Pax9 and Msx1 genetically interact during mouse craniofacial...
BMC Dev Biol - issue: 21 - volume: 14 - pages: .

Theron, A.  et al. 2021

Clinical insights into a tertiary care center cohort of patients with bicuspid aortic valve

Although bicuspid aortic valve (BAV) is one of the most common congenital heart diseases, clinical data associated with valve dysfunction are still limited. We evaluated clinical characteristics and...
Int J Cardiovasc Imaging - issue: - volume: - pages: .

Stefanovic, S.  et al. 2021

Outflow tract formation - Embryonic origins of conotruncal congenital heart disease

Anomalies in the cardiac outflow tract (OFT) are among the most frequent congenital heart defects (CHDs). During embryogenesis, the cardiac OFT is a dynamic structure at the arterial pole of the...
J Cardiovasc Dev Dis. - issue: 8 - volume: 4 - pages: 42.

Faure, E.  et al. 2021

Side-dependent effect in the response of valve endothelial cells to bidirectional shear stress

Endothelial cells covering the aortic and ventricular sides of the aortic valve leaflets are exposed to different stresses, in particular wall shear stress (WSS). Biomechanical stimuli actively...
Int J Cardiol - issue: - volume: 323 - pages: 220-228.

MacGrogan, D.  et al. 2020

Identification of a peripheral blood gene signature predicting aortic valve calcification

Calcific aortic valve disease (CAVD) is a significant cause of illness and death worldwide. Identification of early predictive markers could help optimize patient management. RNA-sequencing was...
Physiol Genomics - issue: 52 - volume: 12 - pages: 563-574.

Stefanovic, S.  et al. 2020

Hox-dependent coordination of mouse cardiac progenitor cell patterning and differentiation

Perturbation of addition of second heart field (SHF) cardiac progenitor cells to the poles of the heart tube results in congenital heart defects (CHD). The transcriptional programs and upstream...
eLife - issue: - volume: 9 - pages: e55124.

Faucherre, A.  et al. 2020

Piezo1 is required for outflow tract and aortic valve development

AIMS: During embryogenesis, the onset of circulatory blood flow generates a variety of hemodynamic forces which reciprocally induce changes in cardiovascular development and performance. It has been...
J Mol Cell Cardio - issue: - volume: 143 - pages: 51-62.

Jaouadi, H.  et al. 2020

Multiallelic rare variants support an oligogenic origin of sudden cardiac death in the young

Unexplained sudden death in the young is cardiovascular in most cases. Structural and conduction defects in cardiac-related genes can conspire to underlie sudden cardiac death. Here we report a...
Herz - issue: - volume: - pages: .

Fultang, L.  et al. 2019

Macrophage-Derived IL1β and TNFα Regulate Arginine Metabolism in Neuroblastoma

Neuroblastoma is the most common childhood solid tumor, yet the prognosis for high-risk disease remains poor. We demonstrate here that arginase 2 (ARG2) drives neuroblastoma cell proliferation via...
Cancer Res. - issue: 3 - volume: 79 - pages: 611-624.

Odelin, G.  et al. 2019

Krox20 Regulates Endothelial Nitric Oxide Signaling in Aortic Valve Development and Disease

Among the aortic valve diseases, the bicuspid aortic valve (BAV) occurs when the aortic valve has two leaflets (cusps), rather than three, and represents the most common form of congenital cardiac...
J Cardiovasc Dev Dis - issue: 4 - volume: 6 - pages: .

Jaouadi, H.  et al. 2019

A severe clinical phenotype of Noonan syndrome with neonatal hypertrophic cardiomyopathy in the second case worldwide with RAF1 S259Y neomutation

Noonan syndrome and related disorders are a group of clinically and genetically heterogeneous conditions caused by mutations in genes of the RAS/MAPK pathway. Noonan syndrome causes multiple...
Genet Res (Camb) - issue: - volume: 101 - pages: e6.

Neri, T.  et al. 2019

Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis

Genetically modified mice have advanced our understanding of valve development and disease. Yet, human pathophysiological valvulogenesis remains poorly understood. Here we report that, by...
Nat Commun - issue: 1 - volume: 10 - pages: 1929.

van Eif, V.  et al. 2019

Transcriptome analysis of mouse and human sinoatrial node cells reveals a conserved genetic program

The rate of contraction of the heart relies on proper development and function of the sinoatrial node, which consists of a small heterogeneous cell population, including Tbx3(+) pacemaker cells. Here,...
Development - issue: - volume: 146 (8) - pages: dev173161.

De Bono, C.  et al. 2018

T-box genes and retinoic acid signaling regulate the segregation of arterial and venous pole progenitor cells in the murine second heart field

The arterial and venous poles of the mammalian heart are hotspots of congenital heart defects (CHD) such as those observed in 22q11.2 deletion (or DiGeorge) and Holt–Oram syndromes. These regions of...
Hum Mol Genet - issue: 21 - volume: 27 - pages: 3747-3760.

Lescroart, F.  et al. 2018

Hox and Tale transcription factors in heart development and disease

Hox genes are highly conserved transcription factors with critical functions during development, in particular for patterning the antero-posterior axis of the embryo. Their action is very often...
Int J Dev Biol - issue: - volume: 62 - pages: 837-846.

Pinard, A.  et al. 2018

Analysis of HOXB1 gene in a cohort of patients with sporadic ventricular septal defect

Ventricular septal defect (VSD) including outlet VSD of double outlet right ventricle (DORV) and perimembranous VSD are among the most common congenital heart diseases found at birth. HOXB1 encodes a...
Mol Biol Rep - issue: 5 - volume: 45 - pages: 1507-1513.

Zaffran, S.  et al. 2018

Ectopic expression of Hoxb1 induces cardiac and craniofacial malformations

Members of the large family of Hox transcription factors are encoded by genes whose tightly regulated expression in development and in space within different embryonic tissues confer positional...
Genesis - issue: 5-6 - volume: 56 - pages: e23221.

Cavodeassi, F.  et al. 2018

The hedgehog pathway and ocular developmental anomalies

Mutations in effectors of the hedgehog signaling pathway are responsible for a wide variety of ocular developmental anomalies. These range from massive malformations of the brain and ocular primordia,...
Hum Genet - issue: - volume: - pages: .

Jaouadi, H.  et al. 2018

Novel ALPK3 mutation in a Tunisian patient with pediatric cardiomyopathy and facio-thoraco-skeletal features

Pediatric cardiomyopathy is a complex disease with clinical and genetic heterogeneity. Recently, the ALPK3 gene was described as a new hereditary cardiomyopathy gene underlying pediatric...
J Hum Genet - issue: - volume: 63 - pages: 1077-1082.

Odelin, G.  et al. 2018

Krox20 defines a subpopulation of cardiac neural crest cells contributing to arterial valves and bicuspid aortic valve

Although cardiac neural crest cells are required at early stages of arterial valve development, their contribution during valvular leaflet maturation remains poorly understood. Here, we show in mouse...
Development - issue: 1 - volume: 145 - pages: pii: dev151944.

Papoutsi, T.  et al. 2018

Bmp2 and Notch cooperate to pattern the embryonic endocardium

Signaling interactions between myocardium and endocardium pattern embryonic cardiac regions, instructing their development to fulfill specific functions in the mature heart. We show that ectopic Bmp2...
Development - issue: 13 - volume: 145 - pages: pii: dev163378..

Métais, A.  et al. 2018

Asb2α-Filamin A Axis Is Essential for Actin Cytoskeleton Remodeling During Heart Development

RATIONALE: Heart development involves differentiation of cardiac progenitors and assembly of the contractile sarcomere apparatus of cardiomyocytes. However, little is known about the mechanisms that...
Circ Res. - issue: 6 - volume: 122 - pages: e34-e48.

Prados, B.  et al. 2018

Myocardial Bmp2 gain causes ectopic EMT and promotes cardiomyocyte proliferation and immaturity

During mammalian heart development, restricted myocardial Bmp2 expression is a key patterning signal for atrioventricular canal specification and the epithelial-mesenchyme transition that gives rise...
Cell Death Dis - issue: 3 - volume: 9 - pages: 399.

Rambeau, P.  et al. 2017

Reduced aggrecan expression affects cardiac outflow tract development in zebrafish and is associated with bicuspid aortic valve disease in humans

Hemodynamic forces have been known for a long time to regulate cardiogenic processes such as cardiac valve development. During embryonic development in vertebrates, the outflow tract (OFT) adjacent to...
Int J Cardiol - issue: - volume: 249 - pages: 340-343.

Ovaert, C.  et al. 2017

FOXC1 haploinsufficiency due to 6p25 deletion in a patient with rapidly progressing aortic valve disease

6p25 deletion is a rare but well-known entity. The main clinical features include an abnormal facial appearance, developmental delay, and ocular anomalies. Cardiac anomalies are frequently seen but...
Am. J. Med. Genet. A - issue: 9 - volume: 173 - pages: 2489-2493.

Labbé, P.  et al. 2017

The alternatively spliced LRRFIP1 Isoform-1 is a key regulator of the Wnt/β-catenin transcription pathway

The GC-rich Binding Factor 2/Leucine Rich Repeat in the Flightless 1 Interaction Protein 1 gene (GCF2/LRRFIP1) is predicted to be alternatively spliced in five different isoforms. Although important...
Biochim. Biophys. Acta - issue: 7 - volume: 1864 - pages: 1142-1152.

Roux, M.  et al. 2017

Hoxa1 and Hoxb1 are required for pharyngeal arch artery development

Hox transcription factors play critical roles during early vertebrate development. Previous studies have revealed an overlapping function of Hoxa1 and Hoxb1 during specification of the rhombomeres...
Mech Dev - issue: - volume: 143 - pages: 1-8.

Stefanovic, S.  et al. 2017

Mechanisms of retinoic acid signaling during cardiogenesis

Substantial experimental and epidemiological data have highlighted the interplay between nutritional and genetic factors in the development of congenital heart defects. Retinoic acid (RA), a...
Mech. Dev. - issue: - volume: 143 - pages: 9-19.

Theron, A.  et al. 2016

An uncommon cause of tricuspid regurgitation: three-dimensional echocardiographic incremental value, surgical and genetic insights

Congenital tricuspid valve disease is a rare defect that includes regurgitation, stenosis and Ebstein's anomaly. We report a case of severe tricuspid regurgitation associated with functional mitral...
Eur. J. Cardio-Thorac. Surg. - issue: 1 - volume: 50 - pages: 180-182.

Roux, M.  et al. 2016

Hox Genes in Cardiovascular Development and Diseases

Congenital heart defects (CHD) are the leading cause of death in the first year of life. Over the past 20 years, much effort has been focused on unraveling the genetic bases of CHD. In particular,...
J. Dev. Biol. - issue: 2 - volume: 4 - pages: 14.

Chassaing, N.  et al. 2016

Targeted resequencing identifies PTCH1 as a major contributor to ocular developmental anomalies and extends the SOX2 regulatory network

Ocular developmental anomalies (ODA) such as anophthalmia/microphthalmia (AM) or anterior segment dysgenesis (ASD) have an estimated combined prevalence of 3.7 in 10,000 births. Mutations in SOX2 are...
Genome Res. - issue: 4 - volume: 26 - pages: 474-485.

El Robrini, N.  et al. 2016

Cardiac outflow morphogenesis depends on effects of retinoic acid signaling on multiple cell lineages

Background: Retinoic acid (RA), the bioactive derivative of vitamin A, is essential for vertebrate heart development. Both excess and reduced RA signaling lead to cardiovascular malformations...
Dev. Dyn. - issue: 3 - volume: 245 - pages: 388-401.

Theron, A.  et al. 2016

Krox20 heterozygous mice: A model of aortic regurgitation associated with decreased expression of fibrillar collagen genes

Background. - The mechanism involved in the onset of aortic valve (AoV) disease remains unclear despite its poor prognosis and frequency. Recently, we reported that Krox20 (EGR2 in humans) is involved...
Arch. Cardiovasc. Dis. - issue: 3 - volume: 109 - pages: 188-198.

Escot, S.  et al. 2016

Disruption of CXCR4 signaling in pharyngeal neural crest cells causes DiGeorge syndrome-like malformations

DiGeorge syndrome (DGS) is a congenital disease causing cardiac outflow tract anomalies, craniofacial dysmorphogenesis, thymus hypoplasia, and mental disorders. It results from defective development...
Development - issue: 4 - volume: 143 - pages: 582-588.

Stefanovic, S.  et al. 2015

GATA-dependent transcriptional and epigenetic control of cardiac lineage specification and differentiation

Heart progenitor cells differentiate into various cell types including pacemaker and working cardiomyocytes. Cell-type specific gene expression is achieved by combinatorial interactions between...
Cell. Mol. Life Sci. - issue: 20 - volume: 72 - pages: 3871-3881.

Roux, M.  et al. 2015

Hoxb1 regulates proliferation and differentiation of second heart field progenitors in pharyngeal mesoderm and genetically interacts with Hoxa1 during cardiac outflow tract development

Outflow tract (OFT) anomalies are among the most common congenital heart defects found at birth. The embryonic OFT grows by the progressive addition of cardiac progenitors, termed the second heart...
Dev. Biol. - issue: 2 - volume: 406 - pages: 247-258.

Papoutsi, T.  et al. 2015

Msx1(creERT2) knock-In allele: A useful tool to target embryonic and adult cardiac valves

Heart valve development begins with the endothelial-to-mesenchymal transition (EMT) of endocardial cells. Although lineage studies have demonstrated contributions from cardiac neural crest and...
Genesis - issue: 5 - volume: 53 - pages: 337-345.

Price, HN.  et al. 2015

Practical Application of the New Classification Scheme for Congenital Melanocytic Nevi

A new consensus-based classification of congenital melanocytic nevi (CMN) has recently been proposed. It includes categories for projected adult size (PAS) and location, satellite nevi counts, and...
Pediatr. Dermatol. - issue: 1 - volume: 32 - pages: 23-27.

Odelin, G.  et al. 2014

Loss of Krox20 results in aortic valve regurgitation and impaired transcriptional activation of fibrillar collagen genes

Aims Heart valve maturation is achieved by the organization of extracellular matrix (ECM) and the distribution of valvular interstitial cells. However, the factors that regulate matrix components...
Cardiovasc. Res. - issue: 3 - volume: 104 - pages: 443-455.

Rana, MS.  et al. 2014

Tbx1 Coordinates Addition of Posterior Second Heart Field Progenitor Cells to the Arterial and Venous Poles of the Heart

Rationale: Cardiac progenitor cells from the second heart field (SHF) contribute to rapid growth of the embryonic heart, giving rise to right ventricular and outflow tract (OFT) myocardium at the...
Circ.Res. - issue: 9 - volume: 115 - pages: 790-U118.

Prados, B.  et al. 2014

Bmp2 patterns prospective valve tissue and regulates EMT and mesenchyme proliferation and morphogenesis

WOS:000341434600199
Transgenic Res. - issue: 5 - volume: 23 - pages: 901-901.

Watanabe, Y.  et al. 2012

Fibroblast growth factor 10 gene regulation in the second heart field by Tbx1, Nkx2-5, and Islet1 reveals a genetic switch for down-regulation in the myocardium

During cardiogenesis, Fibroblast Growth Factor (Fgf10) is expressed in the anterior second heart field. Together with Fibroblast growth factor 8 (Fgf8), Fgf10 promotes the proliferation of these...
Proc. Natl. Acad. Sci. U. S. A. - issue: 45 - volume: 109 - pages: 18273-18280.

Zaffran, S.  et al. 2012

New developments in the second heart field

During cardiac looping the heart tube elongates by addition of progenitor cells from adjacent pharyngeal mesoderm to the arterial and venous poles. This cell population, termed the second heart field,...
Differentiation - issue: 1 - volume: 84 - pages: .

Bun, S.  et al. 2012

Value of In Vivo T2 Measurement for Myocardial Fibrosis Assessment in Diabetic Mice at 11.75 T

Objective: The aim of the study was to assess the value of in vivo T2 measurements to noninvasively quantify myocardial fibrosis in diabetic mice at 11.75 T. Diabetic cardiomyopathy is characterized...
Invest. Radiol. - issue: 5 - volume: 47 - pages: 319-323.

Taylor, J.  et al. 2012

Cardiovascular Research Funding: European Molecular Biology Organization Awards

WOS:000302793300008
Circulation - issue: 14 - volume: 125 - pages: F79-F83.

Golzio, C.  et al. 2012

ISL1 Directly Regulates FGF10 Transcription during Human Cardiac Outflow Formation

The LIM homeodomain gene Islet-1 (ISL1) encodes a transcription factor that has been associated with the multipotency of human cardiac progenitors, and in mice enables the correct deployment of second...
PLoS One - issue: 1 - volume: 7 - pages: e30677.

Diman, NYS.  et al. 2011

A retinoic acid responsive Hoxa3 transgene expressed in embryonic pharyngeal endoderm, cardiac neural crest and a subdomain of the second heart field

A transgenic mouse line harbouring a β-galacdosidase reporter gene controlled by the proximal 2 kb promoter of Hoxa3 was previously generated to investigate the regulatory cues governing Hoxa3...
PLoS ONE - issue: 11 - volume: 6 - pages: e27624.

Bertrand, N.  et al. 2011

Hox genes define distinct progenitor sub-domains within the second heart field

Much of the heart, including the atria, right ventricle and outflow tract (OFT) is derived from a progenitor cell population termed the second heart field (SHF) that contributes progressively to the...
Dev. Biol. - issue: 2 - volume: 353 - pages: 266-274.

Ryckebüsch, L.  et al. 2010

Decreased levels of embryonic retinoic acid synthesis accelerate recovery from arterial growth delay in a mouse model of DiGeorge syndrome

RATIONALE: Loss of Tbx1 and decrease of retinoic acid (RA) synthesis result in DiGeorge/velocardiofacial syndrome (DGS/VCFS)-like phenotypes in mouse models, including defects in septation of the...
Circ. Res. - issue: 4 - volume: 106 - pages: 686-694.

Ryckebusch, L.  et al. 2008

Retinoic acid deficiency alters second heart field formation

Retinoic acid (RA), the active derivative of vitamin A, has been implicated in various steps of cardiovascular development. The retinaldehyde dehydrogenase 2 (RALDH2) enzyme catalyzes the second...
Proc. Natl. Acad. Sci. U.S.A. - issue: 8 - volume: 105 - pages: 2913-2918.