The Genomics and Bioinformatics Marseille (GBiM) core facility is providing state-of-the-art next generation sequencing services in the field of genomics, transcriptomics and epigenetics. We provide services to the academic researchers, as well as private partners.
Our services include genomics, transcriptomics (Bulk, single cell and spatial), epigenomics, as well as bioinformatics analysis of data generated elsewhere.
We have a very broad expertise in the use of Next Generation Sequencing in the field of rare diseases. This include knowledge in designing projects for the study of human samples (DNA, RNA, cells and tissues), as well as all related in vitro and in vivo models for any species.
Our team is accompanying the investigators through all steps of their sequencing projects, from the design of their project to the analysis of their data
We provide any kind of Next Generation Sequencing DNA-Seq for any species and any type of starting biological material.
Below is a list (non-exhaustive) of the projects that you can realize with us:
-Whole Exome Sequencing (WES)
-Whole Genome Sequencing (WGS)
-Targeted exomes or genomes: capture (short read) or adaptive sampling (long read)
- Amplicon sequencing
- Long read plasmid sequencing
We perform both short read and long read (Oxford Nanopore) sequencing.
Bulk RNA-Seq
We perform different types of bulk RNA-Seq:
- mRNA-Seq
- Whole transcriptome sequencing
- Small RNA/miRNA sequencing
- RNA-Seq on native RNA (using Oxford Nanopore)
Single-Cell RNA and spatial transcriptomics:
We are equipped with the Chromium X and Cytassist from10X Genomics and therefore, propose single cell (sc-RNASeq)and spatial transcriptomics, including the latest Visium HD spatial.
We can work from cells, nuclei and tissues fixed or frozen
Here, also, we can perform both long-read and short-read sequencing.
- We can perform ATAC-Seq, ChiP-Seq or other upon demand.
- For methylation, we propose long-read Oxford Nanopore sequencing for both DNA and RNA (direct RNA sequencing).
We support the researchers and clients with tailored analysis for the above cited sequencing. Below is a list of the routine pipelines running in our core facility:
- WES and WGS analysis (short read/long read): SNV, CNV, SV, expansion nucleotide detection, methylation
-RNA-Seq: Differential Gene Expression, Splicing analysis
-scRNA-seq, spatial RNA-Seq
- miRNA-Seq.
This is not an exhaustive list and we are opened to implement new tools to help our clients answering their scientific question.
We perform quality control of your DNA or RNA samples on the 2100 Bioanalyzer or the 4200 Tapestation from Agilent
On demand, we can realize any NGS project. Please don’t hesitate to fill-in the form below to request a quote or a discussion for your project.
Our core facility is equipped with the latest next generation sequencing technologies:
- Short read Sequencing: we use a Illumina Novaseq 6000 platform
- Long read sequencing: gridION Mk1 and PromethION P2 from Oxford Nanopore
- Single Cell transcriptomics: Chromium X from 10X Genomics
- Spatial Transcriptomics: Visium Cystassist from 10X Genomics
-Sample Preparation: Covaris M220 Focused-ultrasonicator
- Pipeting Robot from SPT Labtech for automation of library preparation
- Quality Control: 2100 Bioanalyzer and 4200 Tapestation from Agilent
- High performance computing machines for bioinformatic analyses (CPU and GPU)
Our experiments and analysis are realized according to laboratory best practices (wet and dry) and in conformity with ISO 9001 and NFX-50 standards.
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Telephone: 04 91 32 49 02
Bioinformatics Engineer
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Operation Engineer
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Telephone: 04 91 32 40 26
DR2 , SCIENTIFIC HEAD
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Telephone: 04 91 32 49 02
Bioinformatics Engineer
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Wet Lab Engineer
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Telephone: 04 91 32 49 02
AI
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Telephone: 04 91 32 49 41